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p smad2  (Bioss)


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    Structured Review

    Bioss p smad2
    LPS induced activation of rat uterine fibroblasts. (A) WB detection of LAMC2 expression levels in epithelial cells, (B) Relative expression of LAMC2 in epithelial cells, (C) Level of LAMC2 in cells detected by ELISA in epithelial cells, (D) WB detection of E-cad, N-cad, α-SMA,TGF-β1, <t>SMAD2/3</t> in fibroblasts, (E) Relative expression of E-cad, N-cad, α-SMA,TGF-β1, SMAD2, SMAD3 in fibroblasts, (F) Immunofluorescence staining of COL1A1 in fibroblasts, (G) Immunofluorescence staining of COL3A1 in fibroblasts, (H) Immunofluorescence staining of α-SMA in fibroblasts, (I) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA. Compared to model in fibroblasts, * P < 0.05, ** P < 0.01.
    P Smad2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+p+smad2/Smad2(Ser465+%2B+Ser467)+Antibody/pmc13106170-115-52-56
    Average 94 stars, based on 19 article reviews
    p smad2 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Molecular mechanism of Danshen injection in treating endometrial fibrosis induced by intrauterine adhesions via the LAMC2-CD44-TGF-β1-SMAD2/3 signaling pathway"

    Article Title: Molecular mechanism of Danshen injection in treating endometrial fibrosis induced by intrauterine adhesions via the LAMC2-CD44-TGF-β1-SMAD2/3 signaling pathway

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2026.1794215

    LPS induced activation of rat uterine fibroblasts. (A) WB detection of LAMC2 expression levels in epithelial cells, (B) Relative expression of LAMC2 in epithelial cells, (C) Level of LAMC2 in cells detected by ELISA in epithelial cells, (D) WB detection of E-cad, N-cad, α-SMA,TGF-β1, SMAD2/3 in fibroblasts, (E) Relative expression of E-cad, N-cad, α-SMA,TGF-β1, SMAD2, SMAD3 in fibroblasts, (F) Immunofluorescence staining of COL1A1 in fibroblasts, (G) Immunofluorescence staining of COL3A1 in fibroblasts, (H) Immunofluorescence staining of α-SMA in fibroblasts, (I) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA. Compared to model in fibroblasts, * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: LPS induced activation of rat uterine fibroblasts. (A) WB detection of LAMC2 expression levels in epithelial cells, (B) Relative expression of LAMC2 in epithelial cells, (C) Level of LAMC2 in cells detected by ELISA in epithelial cells, (D) WB detection of E-cad, N-cad, α-SMA,TGF-β1, SMAD2/3 in fibroblasts, (E) Relative expression of E-cad, N-cad, α-SMA,TGF-β1, SMAD2, SMAD3 in fibroblasts, (F) Immunofluorescence staining of COL1A1 in fibroblasts, (G) Immunofluorescence staining of COL3A1 in fibroblasts, (H) Immunofluorescence staining of α-SMA in fibroblasts, (I) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA. Compared to model in fibroblasts, * P < 0.05, ** P < 0.01.

    Techniques Used: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Fluorescence

    Danshen injection improves endometrial fibrosis in IUA rats by inhibiting the LAMC2-CD44-TGF-β1- SMAD2/3 signaling pathway. (A) Uterine Weight, (B) Uterine Length, (C) Grading of intrauterine adhesions in each group, (D) Number of uterine glands, (E) Collagen volume fraction, (F) H&E Staining, (G) MASSON Staining, (H) Immunofluorescence staining of COL1A1, (I) Immunofluorescence staining of COL3A1, (J) Immunofluorescence staining of α-SMA, (K) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA, L: WB detection of LAMC2, TGF-β1, SMAD2, SMAD3, (L) Relative expression of protein LAMC2, TGF-β1, SMAD2, SMAD3. Compared to model, * P < 0.05, ** P < 0.01. For panels A-C, n = 6 independent animals per group; for panels (D–M) , n=3 independent animals per group.
    Figure Legend Snippet: Danshen injection improves endometrial fibrosis in IUA rats by inhibiting the LAMC2-CD44-TGF-β1- SMAD2/3 signaling pathway. (A) Uterine Weight, (B) Uterine Length, (C) Grading of intrauterine adhesions in each group, (D) Number of uterine glands, (E) Collagen volume fraction, (F) H&E Staining, (G) MASSON Staining, (H) Immunofluorescence staining of COL1A1, (I) Immunofluorescence staining of COL3A1, (J) Immunofluorescence staining of α-SMA, (K) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA, L: WB detection of LAMC2, TGF-β1, SMAD2, SMAD3, (L) Relative expression of protein LAMC2, TGF-β1, SMAD2, SMAD3. Compared to model, * P < 0.05, ** P < 0.01. For panels A-C, n = 6 independent animals per group; for panels (D–M) , n=3 independent animals per group.

    Techniques Used: Injection, Staining, Immunofluorescence, Fluorescence, Expressing

    Related Articles

    Western Blot:

    Article Title: MYOC Promotes the Differentiation of C2C12 Cells by Regulation of the TGF-β Signaling Pathways via CAV1
    Article Snippet: .. Antibodies used for Western blotting were: anti-MYOC (1:1000, Sangon, D154098, Shanghai, China), anti-CAV1 (1:1000, Sangon, D261423), anti-MYH2 (1:1000, Santa Cruz Biotechnology, SC-53092, Dallas, TX, USA), anti-MYOG (1:500, Santa, SC-12732), anti-GAPDH (1:2000, Proteintech, 10494-1-AP, Rosemont, IL, USA), anti-Smad2 (1:1000, bs-0718R, Bioss, Beijing, China), anti-P-Smad2 (1:500, bs-7464R, Bioss), anti-Smad4 (1:1000, D120124, Sangon), and anti-P-Smad4 (1:500, Affinity, Ab-AF8316, Jiangsu, China). ..

    Article Title: Hirsutine attenuates renal injury in diabetic kidney disease by regulating Th17 cell differentiation via the TGFβ/SMAD Pathway.
    Article Snippet: Background: Hirsutine (HS), a bioactive indole alkaloid isolated from Uncaria rhynchophylla, a traditional Chinese herb, has long been used in the treatment of hypertension and inflammatory disorders.. However, its nephroprotective potential in diabetic kidney disease (DKD),—a primary cause of end-stage renal failure, remains insufficiently studied, particularly regarding its immunomodulatory mechanisms.. Materials and methods: High-throughput RNA sequencing was performed on renal tissues to identify differentially expressed genes (DEGs).

    Incubation:

    Article Title: Exosomes derived from mesenchymal stem cells reverse EMT via TGF-β1/Smad pathway and promote repair of damaged endometrium
    Article Snippet: .. The primary antibodies of anti-TGF-β1 (1:500; Cat# bs-0086R; Bioss Antibodies, USA), anti-TGF-β1R (1:500; Cat# bs-23446R; Bioss Antibodies, USA), anti-smad2 (1:500; Cat# bs-0718R; Bioss Antibodies, USA), anti-p-smad2 (1:500; Cat# bs-3420R; Bioss Antibodies, USA), CK19 (1:500; Bioss, bs-2190), anti-VIM (1:500; Bioss, bs-8533R), anti- Fibroblast-specific protein-1 (FSP-1) (1:500; Bioss, bs-3759R), anti-E-cadherin (1:500; Bioss, bs-10009R) and anti-GAPDH (1:2000; Cat# bsm330033m, Bioss Antibodies, USA) were added and incubated overnight at 4 °C. ..

    Article Title: Exosomes derived from mesenchymal stem cells reverse EMT via TGF-β1/Smad pathway and promote repair of damaged endometrium.
    Article Snippet: .. The primary antibodies of anti-TGF-β1 (1:500; Cat# bs-0086R; Bioss Antibodies, USA), antiTGF-β1R (1:500; Cat# bs-23446R; Bioss Antibodies, USA), anti-smad2 (1:500; Cat# bs-0718R; Bioss Antibodies, USA), anti-p-smad2 (1:500; Cat# bs-3420R; Bioss Antibodies, USA), CK19 (1:500; Bioss, bs-2190), antiVIM (1:500; Bioss, bs-8533R), anti- Fibroblast-specific protein-1 (FSP-1) (1:500; Bioss, bs-3759R), anti-E-cadherin (1:500; Bioss, bs-10009R) and anti-GAPDH (1: 2000; Cat# bsm330033m, Bioss Antibodies, USA) were added and incubated overnight at 4 °C. ..

    Immunohistochemistry:

    Article Title: Hirsutine attenuates renal injury in diabetic kidney disease by regulating Th17 cell differentiation via the TGFβ/SMAD Pathway.
    Article Snippet: Background: Hirsutine (HS), a bioactive indole alkaloid isolated from Uncaria rhynchophylla, a traditional Chinese herb, has long been used in the treatment of hypertension and inflammatory disorders.. However, its nephroprotective potential in diabetic kidney disease (DKD),—a primary cause of end-stage renal failure, remains insufficiently studied, particularly regarding its immunomodulatory mechanisms.. Materials and methods: High-throughput RNA sequencing was performed on renal tissues to identify differentially expressed genes (DEGs).



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    Image Search Results


    LPS induced activation of rat uterine fibroblasts. (A) WB detection of LAMC2 expression levels in epithelial cells, (B) Relative expression of LAMC2 in epithelial cells, (C) Level of LAMC2 in cells detected by ELISA in epithelial cells, (D) WB detection of E-cad, N-cad, α-SMA,TGF-β1, SMAD2/3 in fibroblasts, (E) Relative expression of E-cad, N-cad, α-SMA,TGF-β1, SMAD2, SMAD3 in fibroblasts, (F) Immunofluorescence staining of COL1A1 in fibroblasts, (G) Immunofluorescence staining of COL3A1 in fibroblasts, (H) Immunofluorescence staining of α-SMA in fibroblasts, (I) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA. Compared to model in fibroblasts, * P < 0.05, ** P < 0.01.

    Journal: Frontiers in Physiology

    Article Title: Molecular mechanism of Danshen injection in treating endometrial fibrosis induced by intrauterine adhesions via the LAMC2-CD44-TGF-β1-SMAD2/3 signaling pathway

    doi: 10.3389/fphys.2026.1794215

    Figure Lengend Snippet: LPS induced activation of rat uterine fibroblasts. (A) WB detection of LAMC2 expression levels in epithelial cells, (B) Relative expression of LAMC2 in epithelial cells, (C) Level of LAMC2 in cells detected by ELISA in epithelial cells, (D) WB detection of E-cad, N-cad, α-SMA,TGF-β1, SMAD2/3 in fibroblasts, (E) Relative expression of E-cad, N-cad, α-SMA,TGF-β1, SMAD2, SMAD3 in fibroblasts, (F) Immunofluorescence staining of COL1A1 in fibroblasts, (G) Immunofluorescence staining of COL3A1 in fibroblasts, (H) Immunofluorescence staining of α-SMA in fibroblasts, (I) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA. Compared to model in fibroblasts, * P < 0.05, ** P < 0.01.

    Article Snippet: Primary antibodies: CK-19 (1:1000; YM8269, Immunoway, USA), Vimentin (1:1000; YM8324, Immunoway, USA), GAPDH (1:1000; A19056, ABclonal, China), LAMC2 (1:1000; A1869, ABclonal, China), E-cad (1:1000; A20798, ABclonal, China), N-cad (1:500; A0433, ABclonal, China), α-SMA (1:1000; A2319, ABclonal, China), TGF-β1 (1:2000; A15103, ABclonal, China), SMAD2 (1:5000; bsm-52223R, Bioss, China), SMAD3 (1:1000; A16913, ABclonal, China), p- SMAD2 (1:5000; bs-3419R, Bioss, China), p- SMAD3 (1:2000; Ap0727, ABclonal, China), and a secondary antibody (1:2000; AS014, ABclonal, China).

    Techniques: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Fluorescence

    Danshen injection improves endometrial fibrosis in IUA rats by inhibiting the LAMC2-CD44-TGF-β1- SMAD2/3 signaling pathway. (A) Uterine Weight, (B) Uterine Length, (C) Grading of intrauterine adhesions in each group, (D) Number of uterine glands, (E) Collagen volume fraction, (F) H&E Staining, (G) MASSON Staining, (H) Immunofluorescence staining of COL1A1, (I) Immunofluorescence staining of COL3A1, (J) Immunofluorescence staining of α-SMA, (K) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA, L: WB detection of LAMC2, TGF-β1, SMAD2, SMAD3, (L) Relative expression of protein LAMC2, TGF-β1, SMAD2, SMAD3. Compared to model, * P < 0.05, ** P < 0.01. For panels A-C, n = 6 independent animals per group; for panels (D–M) , n=3 independent animals per group.

    Journal: Frontiers in Physiology

    Article Title: Molecular mechanism of Danshen injection in treating endometrial fibrosis induced by intrauterine adhesions via the LAMC2-CD44-TGF-β1-SMAD2/3 signaling pathway

    doi: 10.3389/fphys.2026.1794215

    Figure Lengend Snippet: Danshen injection improves endometrial fibrosis in IUA rats by inhibiting the LAMC2-CD44-TGF-β1- SMAD2/3 signaling pathway. (A) Uterine Weight, (B) Uterine Length, (C) Grading of intrauterine adhesions in each group, (D) Number of uterine glands, (E) Collagen volume fraction, (F) H&E Staining, (G) MASSON Staining, (H) Immunofluorescence staining of COL1A1, (I) Immunofluorescence staining of COL3A1, (J) Immunofluorescence staining of α-SMA, (K) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA, L: WB detection of LAMC2, TGF-β1, SMAD2, SMAD3, (L) Relative expression of protein LAMC2, TGF-β1, SMAD2, SMAD3. Compared to model, * P < 0.05, ** P < 0.01. For panels A-C, n = 6 independent animals per group; for panels (D–M) , n=3 independent animals per group.

    Article Snippet: Primary antibodies: CK-19 (1:1000; YM8269, Immunoway, USA), Vimentin (1:1000; YM8324, Immunoway, USA), GAPDH (1:1000; A19056, ABclonal, China), LAMC2 (1:1000; A1869, ABclonal, China), E-cad (1:1000; A20798, ABclonal, China), N-cad (1:500; A0433, ABclonal, China), α-SMA (1:1000; A2319, ABclonal, China), TGF-β1 (1:2000; A15103, ABclonal, China), SMAD2 (1:5000; bsm-52223R, Bioss, China), SMAD3 (1:1000; A16913, ABclonal, China), p- SMAD2 (1:5000; bs-3419R, Bioss, China), p- SMAD3 (1:2000; Ap0727, ABclonal, China), and a secondary antibody (1:2000; AS014, ABclonal, China).

    Techniques: Injection, Staining, Immunofluorescence, Fluorescence, Expressing